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Paper Chromatography Quiz
Test your understanding of paper chromatography with these carefully crafted multiple-choice questions. Designed for 11th grade, this quiz covers principles, techniques, calculations, and common issues in paper chromatography.
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1. What is the primary principle behind paper chromatography?
Explanation: Paper chromatography is based on the partition principle, where the substances separate due to their differing solubilities in the mobile phase and retention in the stationary phase (water molecules bound to the cellulose in the paper).
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2. In paper chromatography, the stationary phase is typically:
Explanation: The stationary phase in paper chromatography is the thin film of water held on the cellulose fibers of the paper.
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3. Which of the following is NOT a type of paper chromatography?
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4. Why should the starting line of the sample in paper chromatography be above the solvent level?
Explanation: If the sample is below the solvent level, it dissolves in the solvent rather than being carried upward for separation.
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5. Retention factor (Rf) is calculated as:
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6. A good Rf value for effective separation typically lies between:
Explanation: Rf values in this range ensure the effective separation of compounds, avoiding overlap or poor resolution.
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7. Which of the following factors does NOT affect Rf values?
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8. Which solvent system is most suitable for separating polar compounds?
Explanation: Polar compounds are better separated using a polar solvent system like a methanol-water mixture.
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9. In two-dimensional paper chromatography, why is the paper rotated by 90° between runs?
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10. What type of compounds are generally difficult to separate using paper chromatography?
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11. Which reagent is commonly used to visualize amino acids separated by paper chromatography?
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12. In paper chromatography, if two solutes have the same Rf value, they:
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13. If a sample spot travels 3 cm and the solvent front travels 6 cm, what is the Rf value?
Answer: (b) 0.5. Rf = (distance traveled by substance) / (distance traveled by solvent front) = 3/6 = 0.5
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14. If two substances have very similar Rf values in one solvent system, what technique can be used to improve separation?
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